Microtox: ammonia and sucrose.
[ MBL's Bioassay/Toxicity Testing Discussion Forum ]
Posted By Marcos Levy on May 08, 2002 at 12:29:19:
Hello. My name is Marcos Levy and i´m a student from Uruguay. I´m making some experiments with the Microtox test. I´m using sucrose as the osmotic pressure adjustment agent, because the effluents i´m trying contain ammonia, and the bibliography says that sucrose raise the sensibility of this test to that ion. But the problem is that when i measure the light emmited by the bacteria at "zero time" the value is too low, so the computer can´t make any calculation. Anyone knows something about it? Anyone has the technique, procedure, SOP, or anything related? Or maybe an alternative to sucrose? Thanks a lot.
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